- cln1 cln2 cln3 Mutants
- cln1∆ cln2∆ cln3∆
- cln1∆ cln2∆ cln3∆ GAL-CLN2
- cln1∆ cln2∆ cln3∆ GAL-CLN3
- cln1∆ cln2∆ cln3∆ sic1∆
- cln1∆ cln2∆ cln3∆ cdh1∆
- cln1∆ cln2∆ cln3∆ multi-copy CLB5
- cln1∆ cln2∆ cln3∆ GAL-CLB5
- cln1∆ cln2∆ cln3∆ multicopy BCK2
- cln1∆ cln2∆ cln3∆ GAL-CLB2
- cln1∆ cln2∆ cln3∆ apc-ts
cln1∆ cln2∆ cln3∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksn2'=ksn2"=0, Dn3=0.
Length of G1 phase: 227 min.
Arrest: G1 arrest according to our rule (see below).
Experiments:
Richardson, H., Wittenberg, C., Cross, F.R. and Reed, S.I. (1989). An essential G1 function for cyclin-like proteins in yeast. Cell 59:1127-1133.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Inviable, G1 arrest.
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A cell must reach [ORI]=1 before the time it takes for a wild type cell in the same medium to divide twice (202.4 min in glucose and 293.4 min in galactose), otherwise it is considered G1-arrested.